解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::We demonstrated for the first time that the COP9 signalosome (COPS) controls the degradation of a surrogate and a bona fide misfolded protein in the cytosol of cardiomyocytes likely via supporting ubiquitination by CUL/cullin-RING ligases, and that Cops8 hypomorphism exacerbates cardiac proteinopathy in mice, in which...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1136773
更新日期:2016-01-01 00:00:00
abstract::Antagonizing the strongly activated pathway of autophagy in renal ischemic injury has been associated with poor outcome. In our recent study we used mice with a selective deletion of Atg5 in the S3 proximal tubule segment, which is most susceptible to ischemic damage. In line with the notion that autophagy is a prosur...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1135284
更新日期:2016-01-01 00:00:00
abstract::Park2/Parkin is a central mediator of selective mitochondrial autophagy for mitochondrial quality control. We showed in mouse hearts that PINK1/Mfn2/Park2 mediated generalized mitophagy is essential to the normal perinatal transition from fetal mitochondria that prefer carbohydrates as metabolic substrates to adult fa...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1143210
更新日期:2016-01-01 00:00:00
abstract::ULK1 (unc-51 like autophagy activating kinase 1), the key mediator of MTORC1 signaling to autophagy, regulates early stages of autophagosome formation in response to starvation or MTORC1 inhibition. How ULK1 regulates the autophagy induction process remains elusive. Here, we identify that ATG13, a binding partner of U...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1140293
更新日期:2016-01-01 00:00:00
abstract::In a recent paper we addressed the mechanism by which defective autophagy contributes to TARDBP/TDP-43-mediated neurodegenerative disorders. We demonstrated that TARDBP regulates MTORC1-TFEB signaling by targeting RPTOR/raptor, a key component and an adaptor protein of MTORC1. Loss of TARDBP decreased the mRNA stabili...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1151596
更新日期:2016-01-01 00:00:00
abstract::Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription factor HsfA1a played a critical role in tomat...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1098798
更新日期:2015-11-02 00:00:00
abstract::ULK1 and ATG13 assemble with RB1CC1/FIP200 and ATG101 to form a macroautophagy (hereafter autophagy) induction (ULK1) complex in higher eukaryotes. The yeast counterpart, the Atg1 complex, is comprised of Atg1 and Atg13 (ULK1 and ATG13 homologs), Atg17 (a proposed functional homolog of RB1CC1), and either the Atg101 s...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1087635
更新日期:2015-11-02 00:00:00
abstract::Here, we present a summary of our recent findings on the (patho-)physiological relevance of PINK1-phosphorylated ubiquitin (p-S65-Ub). Using novel polyclonal antibodies, we find that p-S65-Ub specifically accumulates on damaged mitochondria. Phosphorylation of ubiquitin on serine 65 depends on the activity of PINK1 an...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1091912
更新日期:2015-11-02 00:00:00
abstract::Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and proteins. Impaired autophagic activity is involved in a number of diseases, including idiopathic pulmonary fibrosis suggesting that altered autophagy may contribute to fibrogenesis. However, the specific role of a...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1034409
更新日期:2015-04-03 00:00:00
abstract::ISG15 (ISG15 ubiquitin-like modifier), a ubiquitin-like protein, is one of the major type I IFN (interferon) effector systems. ISG15 can be conjugated to target proteins (ISGylation) via the stepwise action of E1, E2, and E3 enzymes. Conjugated ISG15 can be removed (deISGylated) from target proteins by USP18 (ubiquiti...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1023982
更新日期:2015-04-03 00:00:00
abstract::To maintain proper cellular homeostasis, the magnitude of autophagy activity has to be finely tuned in response to environmental changes. Many aspects of autophagy regulation have been extensively studied: pathways integrating signals through the master regulators TORC1 and PKA lead to multiple post-translational modi...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1018503
更新日期:2015-04-03 00:00:00
abstract::Cocaine abuse leads to neuroinflammation, which, in turn, contributes to the pathogenesis of neurodegeneration associated with advanced HIV-1 infection. Autophagy plays important roles in both innate and adaptive immune responses. However, the possible functional link between cocaine and autophagy has not been explore...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1052205
更新日期:2015-01-01 00:00:00
abstract::Macroautophagy is an intracellular catabolic process involved in the formation of multiple membrane structures ranging from phagophores to autophagosomes and autolysosomes. Dysfunction of macroautophagy is implicated in both physiological and pathological conditions. To date, 38 autophagy-related (ATG) genes have been...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/15548627.2014.984267
更新日期:2015-01-01 00:00:00
abstract::Oxidative stress (OS) stimulates autophagy in different cellular systems, but it remains controversial if this rule can be generalized. We have analyzed the effect of chronic OS induced by the parkinsonian toxin paraquat (PQ) on autophagy in astrocytoma cells and primary astrocytes, which represent the first cellular ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1058683
更新日期:2015-01-01 00:00:00
abstract::The lack of a rapid and quantitative autophagy assay has substantially hindered the development and implementation of autophagy-targeting therapies for a variety of human diseases. To address this critical issue, we developed a novel autophagy assay using the newly developed Cyto-ID fluorescence dye. We first verified...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1017181
更新日期:2015-01-01 00:00:00
abstract::Cigarette smoke (CS)-induced mitochondrial damage with increased reactive oxygen species (ROS) production has been implicated in COPD pathogenesis by accelerating senescence. Mitophagy may play a pivotal role for removal of CS-induced damaged mitochondria, and the PINK1 (PTEN-induced putative kinase 1)-PARK2 pathway h...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1017190
更新日期:2015-01-01 00:00:00
abstract::Adaptive immunity, which plays an important role in the development of atherosclerosis, is mediated by major histocompatibility complex (MHC)-dependent antigen presentation. In atherosclerotic lesions, macrophages constitute an important class of antigen-presenting cells that activate adaptive immune responses to oxid...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1037061
更新日期:2015-01-01 00:00:00
abstract::In liver the mitochondrial sirtuin, SIRT5, controls ammonia detoxification by regulating CPS1, the first enzyme of the urea cycle. However, while SIRT5 is ubiquitously expressed, urea cycle and CPS1 are only present in the liver and, to a minor extent, in the kidney. To address the possibility that SIRT5 is involved i...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1009778
更新日期:2015-01-01 00:00:00
abstract::Recent evidence has indicated that the lysosome is able to act as a signaling organelle that senses nutrient availability and generates an adaptive response that is important for cellular homeostasis. We recently discovered another example of lysosomal signaling where lysosomal calcium release activates the master aut...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.1080/15548627.2015.1047130
更新日期:2015-01-01 00:00:00
abstract::In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/15548627.2014.994413
更新日期:2015-01-01 00:00:00
abstract::Autophagosomes arise in yeast and animals from the sealing of a cup-shaped double-membrane precursor, the phagophore. The concerted action of about 30 evolutionarily conserved autophagy related (ATG) proteins lies at the core of this process. However, the mechanisms allowing phagophore generation and its differentiati...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29962
更新日期:2014-10-01 00:00:00
abstract::It has not been elucidated whether or not autophagy is induced by rhabdoviral G glycoproteins (G) in vertebrate organisms for which rhabdovirus infection is lethal. Our work provides the first evidence that both mammalian (vesicular stomatitis virus, VSV) and fish (viral hemorrhagic septicemia virus, VHSV, and spring ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29557
更新日期:2014-09-01 00:00:00
abstract::Autophagy and microRNA (miRNA) are important regulators during cancer cell tumorigenesis. Impaired autophagy and high expression of the oncogenic microRNA MIR224 are prevalent in hepatocellular carcinoma (HCC); however, the relationship between the 2 phenomena remains elusive. In this study, we are the first to reveal...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29959
更新日期:2014-09-01 00:00:00
abstract::We have recently shown the roles of an autophagy gene in the regulation of metabolism and metabolic diseases. We identified Becn2/Beclin 2, a novel mammalian specific homolog of Becn1/Beclin 1, characterized the functions of the gene product in autophagy and agonist-induced lysosome-mediated downregulation of a subset...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29414
更新日期:2014-08-01 00:00:00
abstract::Preclinical studies indicate autophagy inhibition with hydroxychloroquine (HCQ) can augment the efficacy of DNA-damaging therapy. The primary objective of this trial was to determine the maximum tolerated dose (MTD) and efficacy of HCQ in combination with radiation therapy (RT) and temozolomide (TMZ) for newly diagnos...
journal_title:Autophagy
pub_type: 杂志文章,多中心研究
doi:10.4161/auto.28984
更新日期:2014-08-01 00:00:00
abstract::We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rather than autophagosom...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29394
更新日期:2014-07-01 00:00:00
abstract::Glioblastoma is one of the most aggressive human cancers with poor prognosis, and therefore a critical need exists for novel therapeutic strategies for management of glioblastoma patients. Itraconazole, a traditional antifungal drug, has been identified as a novel potential anticancer agent due to its inhibitory effec...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.28912
更新日期:2014-07-01 00:00:00
abstract::The islet in type 2 diabetes mellitus (T2DM) is characterized by a deficit in β-cells and increased β-cell apoptosis attributable at least in part to intracellular toxic oligomers of IAPP (islet amyloid polypeptide). β-cells of individuals with T2DM are also characterized by accumulation of polyubiquitinated proteins ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.28478
更新日期:2014-06-01 00:00:00
abstract::The multifaceted process of aging inevitably leads to disturbances in cellular metabolism and protein homeostasis. To meet this challenge, cells make use of autophagy, which is probably one of the most important pathways preserving cellular protection under stressful conditions. Thus, efficient autophagic flux is requ...
journal_title:Autophagy
pub_type: 评论,杂志文章
doi:10.4161/auto.28767
更新日期:2014-06-01 00:00:00
abstract::It has been a longstanding problem to identify specific and efficient pharmacological modulators of autophagy. Recently, we found that depletion of acetyl-coenzyme A (AcCoA) induced autophagic flux, while manipulations designed to increase cytosolic AcCoA efficiently inhibited autophagy. Thus, the cell permeant ester ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.28235
更新日期:2014-05-01 00:00:00
abstract::Macroautophagy is a complex process involving dynamic membrane rearrangements in which parts of the cytoplasm are sequestered within double-membrane phagophores. Upon completion, these structures mature into autophagosomes that fuse with the yeast vacuole or mammalian lysosome, leading to degradation of the cargo and ...
journal_title:Autophagy
pub_type: 社论
doi:10.4161/auto.27965
更新日期:2014-05-01 00:00:00
abstract::The highly invasive and chemoresistant phenotype of pancreatic cancer highlights the urgency to identify prognostic biomarkers and novel therapeutic targets. Recently, we observed a significant correlation between shorter survival and loss of the cytoband 18q22.3. Here we investigated genes encoded by this cytoband, a...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.27803
更新日期:2014-04-01 00:00:00
abstract::T lymphocytes, the master regulators of immunity, have an unusual lifestyle. Equipped with a clonally distributed receptor they remain resting for long periods of time but go into overdrive when encountering antigen. Antigen recognition triggers an activation program that results in massive proliferation, differentiat...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.27345
更新日期:2014-02-01 00:00:00
abstract::Autophagy is a highly conserved homeostatic pathway that plays an important role in tumor development and progression by acting on cancer cells in a cell-autonomous mechanism. However, the solid tumor is not an island, but rather an ensemble performance that includes nonmalignant stromal cells, such as macrophages. A ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.26927
更新日期:2014-02-01 00:00:00
abstract::The aging process drives the progressive deterioration of an organism and is thus subject to a complex interplay of regulatory and executing mechanisms. Our understanding of this process eventually aims at the delay and/or prevention of age-related pathologies, among them the age-dependent decrease in cognitive perfor...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.26918
更新日期:2014-01-01 00:00:00
abstract::Recent evidence suggests that autophagy may favor fibrosis through enhanced differentiation of fibroblasts in myofibroblasts. Here, we sought to characterize the mediators and signaling pathways implicated in autophagy-induced myofibroblast differentiation. Fibroblasts, serum starved for up to 4 d, showed increased LC...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/15548627.2014.981786
更新日期:2014-01-01 00:00:00
abstract::The conserved target of rapamycin (TOR) kinase is a central regulator of cell growth in response to nutrient availability. TOR forms 2 structurally and functionally distinct complexes, TORC1 and TORC2, and negatively regulates autophagy via TORC1. Here we demonstrate TOR also operates independently through the TORC2 s...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.36262
更新日期:2014-01-01 00:00:00
abstract::Silver nanoparticles (Ag NPs) are cytotoxic to cancer cells and possess excellent potential as an antitumor agent. A variety of nanoparticles have been shown to induce autophagy, a critical cellular degradation process, and the elevated autophagy in most of these situations promotes cell death. Whether Ag NPs can indu...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.36293
更新日期:2014-01-01 00:00:00
abstract::Delivery of cellular contents to yeast vacuoles/mammalian lysosomes via autophagy ensures long-term cell survival and extends life span. When cultured yeast cells are grown for a prolonged period of time to enter stationary phase, a nondividing state mimicking quiescence, vacuolar membrane proteins partition into eith...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.36137
更新日期:2014-01-01 00:00:00
abstract::SNCA/α-synuclein aggregation plays a crucial role in synucleinopathies such as Parkinson disease and dementia with Lewy bodies. Aggregating and nonaggregating SNCA species are degraded by the autophagy-lysosomal pathway (ALP). Previously, we have shown that the ALP is not only responsible for SNCA degradation but is a...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.36436
更新日期:2014-01-01 00:00:00